Systematically Reviewing The High-Performance Liquid Chromatography (HPLC) Analytical Methods For Determining Empagliflozin In Different Pharmaceutical Formulations And Human Samples

Abstract

Empagliflozin is an oral sodium-glucose cotransporter 2 (SGLT2) inhibitor. Chemically, empagliflozin belongs to C-glucosides, and it works by potently blocking glucose reabsorption in the proximal tubule, increasing urinary glucose excretion. Several methods have been reported in an industrial scale for thorough quantitative analysis. This review article comprehensively examines the published sophisticated instruments-based nearly 30 analytical methods in various pharmaceutical databases like Google Scholar, PubMed, etc. of diverse area of High-Performance Liquid Chromatography (HPLC). The determination of Empagliflozin in plasma, serum, and urine, extensively requires the HPLC method. For the determination of Empagliflozin in biological samples, we recommend the LC-MS/MS method since this method combines the HPLC separation ability with MS sensitivity and selectivity, allowing the identification of Empagliflozin and its metabolites. This fascinating review comprehensively highlighted the overview of sophisticated analytical techniques for the method development and validation for Empagliflozin in the most reliable, accurate, precise, economic, reproducible, and robust manner.